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Filter Sets by Fluorophore Reference

This is a page which contains an alphabetical listing of commonly used single-dye applications. Alongside are the recommended filter sets to use for each dye.
If you cannot find your particular fluorophore in this listing, please contact as we will be able to advise you of the most suitable filters to use.

Filter Sets by Part Number

Attached is a complete listing of filter sets by stock number, including single-band, multi-band and other application specific stock sets.
Also available on-line are the spectra of each filter set.

Vivid Classic Sets

These are the basic filter sets and are designed primarily for viewing single fluorophores. Excitation filters are bandpass designs. Dichroics are hard surface coated. Emission filters are longpass designs manufactured from Schott absorption glass. Vivid Classic sets perform well for single stained samples with low auto-fluorescence. Signal-to-noise is not as high as in other sets.

Vivid Standard Sets

These are the standard filter sets which provide good overall performance for most fluorescent dyes. Excitation filters are bandpass designs. Dichroics are hard surface coated. The emission filter is a bandpass design, replacing the longpass emission filter of the Vivid Classic Sets. This design transmits the prominence of the fluorophore signal while blocking unwanted background energy. These sets provide less overall net throughput than the ALPHA Vivid Sets.

Vivid Plus Sets

These are the filter sets that utilise Alpha Technology for the emission filters. This results in much steeper edges than any other filter manufacturing method, while increasing attenuation and virtually eliminating auto-fluorescence. Excitation filters use a bandpass filter design as in Vivid Class and Vivid Standard sets. Dichroics are hard surface coated. Emission filters are ALPHA longpass designs. Vivid Plus sets provide the brightest images for most fluorescent dyes, with less colour definition than the ALPHA Vivid Sets.

Alpha Vivid Sets

ALPHA Vivid sets use proprietary APLHA technology for both the excitation and the emission filters. Passband slopes are extremely steep, with transmission approaching unity in both the excitation and emission channels. The crossover between excitation and emission has the highest optical density (OD) of any available filter sets. ALPHA Vivid sets are manufactured without auto-fluorescent absorption glass typically used in manufacturing filters. ALPHA Vivid sets are listed for commonly used fluorophores and can be custom designed for any dye. With extreme edge steepness, high throughput, and high density crossover blocking, ALPHA Vivid sets are the current state-of-the-art for signal-to-noise in low light level imaging applications.

Multiband Filter Sets

Multi-band sets are used for multiple labelling and simultaneous viewing of multiple fluorophores. Each set of exciters, dichroics, and emitters yields isolated bands of excitation and emission energy.
Stock sets include dual, triple and quadruple bandpass designs. Each exciter and emitter filter contains multiple passbands. Multiband sets are available for a variety of applications, such as fluorescence in-situ hybridisation (FISH) and immunolabelling.

Pinkel Filter Sets

Pinkel Sets utilise the dichroic and emitter from the multi-band sets along with a series of exciters instead of the multi-band option to create composite images. The advantages of using the Pinkel Sets are as follows: the exposure of each colour can be individually optimised; the imaging is performed one at a time, eliminating the need for a colour detector; and a composite image is achieved.


DsRed Sets

DsRed Sets are designed to work with the new red fluorescent protein (RFP) mutant. The sets are designed to work with either a black & white detector, or for colour film and visualisation by eye.

Fluorescent Protein Sets (GFP, RFP, CFP, YFP)

These filters sets are designed exclusively for GFP spectral mutants. Since their original cloning, these unique reporters have allowed researchers to visualise gene expression and intracellular protein localisation in living cells. With the introduction of optimised spectral variants of fluorescent proteins, researchers now have the flexibility to use multiple fluorescent protein reporters.


These sets are designed for Fluorescent Resonant Energy Transfer, a technique for determining distance in a sample. FRET using GFP spectral mutants provides the ability to localise and monitor ion-binding and molecular protein-protein interactions in living cells. We offer sets for ratio-metric measurement of CFP/YFP, BFP/GFP, and GFP/DsRed FRET emission and are continuing development of the FRET filters sets for emerging applications.

Fluorescent Protein Multiband Sets

Multi-band sets allow researchers to simultaneously visualise spectral variants of fluorescent proteins. Individual exciters for these sets are also available.

3rd Millennium Filter Sets

The latest technological advance; 3rd Millennium filters combine a patented, hermetic physical assembly with proprietary ALPHA Technology. A process which produces exceptionally steep cut-on and cut-off slopes. This results in more precise location of cut-on and cut-off points, the ability to place transmission and rejection regions extremely close together, and higher attenuation between the pass band and the rejection band.
All this results in a brighter sharper image with a blacker background, while having the ability to tune the filter cut-on and cut-offs to your requirements.

Quantum dot filter Sets

These filter sets have been developed and optimized for imaging of Qdot semi-conductor nanocrystals. These filter sets use emission filters centred on the quantum dot emission centre wavelengths. Th result is bright signal and excellent signal-to-noise at an economical price. This design starategy is possible as a result of the karge Stokes shift inherent in quantum dot technology.


M-Fish Filter Sets

M-FISH applications are using an increasing number of colour probes. To maximise multi-colour labelling, we have organized fluorophores into 10 different colour groupsings and have designed sets for each group. The edges are steep and the bandwidth is narrow allowing maximisation of both the emission energy and the colour discrimination. These sets are recommended for CGH, SKY and Co-localizarion studies.

Kaede Filter Sets

The Kaede filter set for fluoresence real time imaging use an optical masking technique useful in studying cell dynamics. Fluoresence from Kaede, a coral protein, changes from bright green to bright red when exposed to UV light. This filter set accommodates both green and red fluoresences as well as the UV conversion. this set has been designed in collaboration with Atsushi Miyawaki et al.


Sedat Sets

The Sedat filter sets are recommended for multi-colour applications abd result in minimal image shift and increased signal-to-noise in comparision to standard multi-band and Pinkel sets. Single band excitation and emission filters are normally installed in automated filter wheels with a stationary dichroic in the microscope for quick colour changes. these sets were first conceived by Dr. John Sedat at UCSF.


Fluorescence Reference Slides

Fluor-Ref slides provide a quick and easy to use method of calibrating and standardising fluorescence imaging systems. This set of slides helps to: centre and adjust the fluorescence illuminator, verify uniformity of fluorescence staining, monitor and adjust laser output and PMT settings, and avoid microspheres and photobleaching.
The Set contains four slides with spectra approximating the most commonly used fluorophores: DAPI (Blue emission, FITC/GFP (Green Emission), Acridine Orange (Yellow emission), Rhodamine/Texas Red (Red emission).


Ratio Imaging Sets

Single-dye Ratio imaging sets are designed to achieve sensitive measurements of the listed indicator dyes and are divided into excitation and emission ratio imaging. If your application requires measurement at the dye's isosbestic point as a control, contact our application specialists.

Caged Compound Sets

Caged Compound sets use extended reflection dichroics, enabling researchers to use both an uncaging filter and an exciter with the same dichroic. Both filters are mounted in a motorised filter wheel in the excitation path so that imaging quality can follow immediately after uncaging. The dichroic, as well as a specific emission filter for the fluorophore being uncaged, is housed in a conventional filter holder.

Fluorophore Reference Table

Filter Sets




Filter Holders

Neutral Density Filters

Accessory Filters

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