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Sets by Fluorophore Reference
This is a
page which contains an alphabetical listing of commonly used
single-dye applications. Alongside are the recommended filter sets to
use for each dye.
cannot find your particular fluorophore in this listing, please
we will be able to advise you of the most suitable filters to use.
Sets by Part Number
a complete listing of filter sets by stock number, including
single-band, multi-band and other application specific stock sets.
available on-line are the spectra of each filter set.
the basic filter sets and are designed primarily for viewing single
fluorophores. Excitation filters are bandpass designs. Dichroics are
hard surface coated. Emission filters are longpass designs
manufactured from Schott absorption glass. Vivid Classic sets perform
well for single stained samples with low auto-fluorescence.
Signal-to-noise is not as high as in other sets.
the standard filter sets which provide good overall performance for
most fluorescent dyes. Excitation filters are bandpass designs.
Dichroics are hard surface coated. The emission filter is a bandpass
design, replacing the longpass emission filter of the Vivid Classic
Sets. This design transmits the prominence of the fluorophore signal
while blocking unwanted background energy. These sets provide less
overall net throughput than the ALPHA Vivid Sets.
the filter sets that utilise Alpha Technology for the emission
filters. This results in much steeper edges than any other filter
manufacturing method, while increasing attenuation and virtually
eliminating auto-fluorescence. Excitation filters use a bandpass
filter design as in Vivid Class and Vivid Standard sets. Dichroics
are hard surface coated. Emission filters are ALPHA longpass designs.
Vivid Plus sets provide the brightest images for most fluorescent
dyes, with less colour definition than the ALPHA Vivid Sets.
sets use proprietary APLHA technology for both the excitation and the
emission filters. Passband slopes are extremely steep, with
transmission approaching unity in both the excitation and emission
channels. The crossover between excitation and emission has the
highest optical density (OD) of any available filter sets. ALPHA
Vivid sets are manufactured without auto-fluorescent absorption glass
typically used in manufacturing filters. ALPHA Vivid sets are listed
for commonly used fluorophores and can be custom designed for any
dye. With extreme edge steepness, high throughput, and high density
crossover blocking, ALPHA Vivid sets are the current state-of-the-art
for signal-to-noise in low light level imaging applications.
sets are used for multiple labelling and simultaneous viewing of
multiple fluorophores. Each set of exciters, dichroics, and emitters
yields isolated bands of excitation and emission energy.
include dual, triple and quadruple bandpass designs. Each exciter and
emitter filter contains multiple passbands. Multiband sets are
available for a variety of applications, such as fluorescence in-situ
hybridisation (FISH) and immunolabelling.
utilise the dichroic and emitter from the multi-band sets along with
a series of exciters instead of the multi-band option to create
composite images. The advantages of using the Pinkel Sets are as
follows: the exposure of each colour can be individually optimised;
the imaging is performed one at a time, eliminating the need for a
colour detector; and a composite image is achieved.
are designed to work with the new red fluorescent protein (RFP)
mutant. The sets are designed to work with either a black & white
detector, or for colour film and visualisation by eye.
Protein Sets (GFP, RFP, CFP, YFP)
filters sets are designed exclusively for GFP spectral mutants. Since
their original cloning, these unique reporters have allowed
researchers to visualise gene expression and intracellular protein
localisation in living cells. With the introduction of optimised
spectral variants of fluorescent proteins, researchers now have the
flexibility to use multiple fluorescent protein reporters.
are designed for Fluorescent Resonant Energy Transfer, a technique
for determining distance in a sample. FRET using GFP spectral mutants
provides the ability to localise and monitor ion-binding and
molecular protein-protein interactions in living cells. We offer sets
for ratio-metric measurement of CFP/YFP, BFP/GFP, and GFP/DsRed FRET
emission and are continuing development of the FRET filters sets for
Protein Multiband Sets
sets allow researchers to simultaneously visualise spectral variants
of fluorescent proteins. Individual exciters for these sets are also available.
Millennium Filter Sets
technological advance; 3rd Millennium filters combine a patented,
hermetic physical assembly with proprietary ALPHA Technology. A
process which produces exceptionally steep cut-on and cut-off slopes.
This results in more precise location of cut-on and cut-off points,
the ability to place transmission and rejection regions extremely
close together, and higher attenuation between the pass band and the
results in a brighter sharper image with a blacker background, while
having the ability to tune the filter cut-on and cut-offs to your requirements.
dot filter Sets
filter sets have been developed and optimized for imaging of Qdot
semi-conductor nanocrystals. These filter sets use emission filters
centred on the quantum dot emission centre wavelengths. Th result is
bright signal and excellent signal-to-noise at an economical price.
This design starategy is possible as a result of the karge Stokes
shift inherent in quantum dot technology.
applications are using an increasing number of colour probes. To
maximise multi-colour labelling, we have organized fluorophores into
10 different colour groupsings and have designed sets for each group.
The edges are steep and the bandwidth is narrow allowing maximisation
of both the emission energy and the colour discrimination. These sets
are recommended for CGH, SKY and Co-localizarion studies.
filter set for fluoresence real time imaging use an optical masking
technique useful in studying cell dynamics. Fluoresence from Kaede, a
coral protein, changes from bright green to bright red when exposed
to UV light. This filter set accommodates both green and red
fluoresences as well as the UV conversion. this set has been designed
in collaboration with Atsushi Miyawaki et al.
filter sets are recommended for multi-colour applications abd result
in minimal image shift and increased signal-to-noise in comparision
to standard multi-band and Pinkel sets. Single band excitation and
emission filters are normally installed in automated filter wheels
with a stationary dichroic in the microscope for quick colour
changes. these sets were first conceived by Dr. John Sedat at UCSF.
slides provide a quick and easy to use method of calibrating and
standardising fluorescence imaging systems. This set of slides helps
to: centre and adjust the fluorescence illuminator, verify uniformity
of fluorescence staining, monitor and adjust laser output and PMT
settings, and avoid microspheres and photobleaching.
contains four slides with spectra approximating the most commonly
used fluorophores: DAPI (Blue emission, FITC/GFP (Green Emission),
Acridine Orange (Yellow emission), Rhodamine/Texas Red (Red emission).
Ratio imaging sets are designed to achieve sensitive measurements of
the listed indicator dyes and are divided into excitation and
emission ratio imaging. If your application requires measurement at
the dye's isosbestic point as a control, contact our application specialists.
Compound sets use extended reflection dichroics, enabling researchers
to use both an uncaging filter and an exciter with the same dichroic.
Both filters are mounted in a motorised filter wheel in the
excitation path so that imaging quality can follow immediately after
uncaging. The dichroic, as well as a specific emission filter for the
fluorophore being uncaged, is housed in a conventional filter holder.